COXII Antikörper

Produktnummer ABIN334535

Der Kaninchen Polyklonal Anti-COXII-Antikörper wurde für WB und BN PAGE validiert. Er ist geeignet, COXII in Proben von Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild Cabbage, Chickpea, Nicotiana tabacum und Mais (Zea mays) zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für COXII Antikörper (ABIN334535)

Target: COXII (Cytochrome Oxidase Subunit II (COXII))

Reaktivität: Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild Cabbage, Chickpea, Nicotiana tabacum, Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COXII Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Blue-native PAGE (BN PAGE)

Produktdetails anti-COXII Antikörper

Spezifität: Arabidopsis thaliana (leaf extract and isolated mitochondria).

Homologie: dicots including Cucumis melo, Glycine max, Pisum sativum, monocots including Oryza sativa, moss Physcomitrella patens

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 29.4 / 30 kDa (for Arabidopsis thaliana)

Aufreinigung: serum

Immunogen: KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots includingArabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9

Anwendungsinformationen

Applikationshinweise: Recommended Dilution 1 : 1000 (WB), 1 : 1000 (BN-PAGE).

Kommentare:

antibody detects COXII protein in total leaf extracts and isolated mitochondriaBlue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen für anti-COXII Antikörper (ABIN334535)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).

Hopff, Wienkoop, Lüthje: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).

Miller, Grant, Giles, Ribas-Carbo, Berry, Watling, Robinson: "In the heat of the night - alternative pathway respiration drives thermogenesis in Philodendron bipinnatifidum." in: The New phytologist, (2010) (PubMed).

Details zu COXII

Target: COXII (Cytochrome Oxidase Subunit II (COXII))

Andere Bezeichnung: COXII

Hintergrund: AGI Code: ATMG00160
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

Molekulargewicht: expected: 29.4 kDa, apparent: 30 kDa (for Arabidopsis thaliana)

UniProt: Q1XGA9, P04373