COXII Antikörper

Produktnummer ABIN334535

Es sind 4+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-COXII Antikörper wird verwendet zum Nachweis von COXII in Proben von Cabbage, Physcomytrium Patens, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Arabidopsis thaliana, durum wheat, Mais (Zea mays), Vicia faba, Triticum aestivum, Rape, Quercus rubra, Picea abies, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Eriophorum vaginatum, Cucumis sativus, Cucumis melo, Chickpea, Betula nana, Gerste und Actinidia chinensis. Er wurde validiert für WB und BN PAGE.

Kurzübersicht für COXII Antikörper (ABIN334535)

Target: COXII (Cytochrome Oxidase Subunit II (COXII))

Reaktivität: Cabbage, Physcomytrium Patens, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Arabidopsis thaliana, durum wheat, Mais (Zea mays), Vicia faba, Triticum aestivum, Rape, Quercus rubra, Picea abies, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Eriophorum vaginatum, Cucumis sativus, Cucumis melo, Chickpea, Betula nana, Gerste, Actinidia chinensis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COXII Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Blue-native PAGE (BN PAGE)

Produktdetails anti-COXII Antikörper

Verwendungszweck: Anti-COXII | Plant Cytochrome oxidase subunit II (affinity purified)

Spezifität: Arabidopsis thaliana (leaf extract and isolated mitochondria).

Kreuzreaktivität (Details): Actinidia chinensis, Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Cucumis sativus, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrium patens, Triticum aestivum, Triticum durum Desf., Zea mays, Vicia faba, Quercus rubra
Not reactive in: Mangifera indica, Saccharina japonica

Homologie: Cucumis melo,Glycine max,Gossypium hirsutum,Neurachne alopecuroidea,Neurachne minor,Neurachne muelleri,Oryza sativa,Physcomitrium patens,Pisum sativum,Triticum aestivum,Vigna radiata

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160

Anwendungsinformationen

Applikationshinweise: 1 : 1000 (BN-PAGE), 1 : 1000 (WB)

Kommentare:

antibody detects COXII protein in total leaf extracts and isolated mitochondriaBlue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-COXII Antikörper (ABIN334535)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).

Hopff, Wienkoop, Lüthje: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).

Miller, Grant, Giles, Ribas-Carbo, Berry, Watling, Robinson: "In the heat of the night - alternative pathway respiration drives thermogenesis in Philodendron bipinnatifidum." in: The New phytologist, (2010) (PubMed).

Details zu COXII

Target: COXII (Cytochrome Oxidase Subunit II (COXII))

Andere Bezeichnung: COXII

Hintergrund:

Background: Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

Additional information: Cellular [compartment marker] of mitochondrial inner membrane

Molekulargewicht: 29.4 | 30 kDa (for Arabidopsis thaliana)

Gen-ID: 3989052

UniProt: P04373, Q1XGA9