HNF1B Antikörper (Internal Region)

Produktnummer ABIN334505

Der Ziege Polyklonal Anti-HNF1B-Antikörper wurde für WB, ELISA und ICC validiert. Er ist geeignet, HNF1B in Proben von Human und Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für HNF1B Antikörper (Internal Region) (ABIN334505)

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HNF1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Güteklasse: Verified

Produktdetails anti-HNF1B Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: TCF2 / VHNF1

Sequenz: QAYDRQKNPS KEER

Spezifität: This antibody is expected to recognize both reported isoforms (NP_000449.1, NP_001159395.1). This product may cross-react with HNF1A.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte, Zebrafisch (Danio rerio)

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QAYDRQKNPSKEER, from the internal region of the protein sequence according to NP_000449.1, NP_001159395.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 60 kDa band observed in Human Liver lysates (calculated MW of 61.3 kDa according to NP_000449.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently ob

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunocytochemistry: A customer found positive results in ICC on Human primitive gut tube from Human embryonic stem cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-HNF1B Antikörper (ABIN334505)

Arenas, Hervias, Uriz, Joplin, Prieto, Medina: "Combination of ursodeoxycholic acid and glucocorticoids upregulates the AE2 alternate promoter in human liver cells." in: The Journal of clinical investigation, Vol. 118, Issue 2, pp. 695-709, (2008) (PubMed).

Details zu HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Andere Bezeichnung: HNF1B

Hintergrund: TCF2, VHNF1, transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor, FJHN, HNF1B, HNF1beta, HNF2, LFB3, MODY5, transcription factor 2

Gen-ID: 6928, 21410, 25640

NCBI Accession: NP_000449, NP_001159395

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation