s100a4 Antikörper (C-Term)

Produktnummer ABIN334485

Dieses Anti-s100a4-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von s100a4 in WB, IHC und ELISA. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für s100a4 Antikörper (C-Term) (ABIN334485)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser s100a4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-s100a4 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: S100A4 / CAPL

Sequenz: CNEFFEGFPD KQP

Spezifität: Both variants (NP_002952.1, NP_062427.1) represent identical protein.

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CNEFFEGFPDKQP, from the C Terminus of the protein sequence according to NP_002952.1, NP_062427.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of lymphatic cells in the lamina propria. Recommended concentration: 1-2 μg/mL.

Western Blot: Approx 13 kDa band observed in Human Peripheral blood mononuclear cells lysates (calculated MW of 11.7 kDa according to NP_002952.1). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-s100a4 Antikörper (ABIN334485)

Rasanen, Sriswasdi, Valiga, Tang, Zhang, Perego, Somasundaram, Li, Speicher, Klein-Szanto, Basu, Rustgi, Speicher, Herlyn: "Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 12, pp. 3778-92, (2013) (PubMed).

Details zu s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Andere Bezeichnung: S100A4

Hintergrund: S100A4, CAPL, S100 calcium binding protein A4, 18A2, 42A, MTS1, P9KA, PEL98, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S100 calcium-binding protein A4 (calcium pr

Gen-ID: 6275, 20198, 24615

NCBI Accession: NP_002952, NP_062427

Pathways: S100 Proteine