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IDE Antikörper (Internal Region)

Dieser Anti-IDE Antikörper ist ein Ziege Polyklonal Antikörper zur Detektion von IDE in WB, IHC und ELISA. Geeignet für Human.
Produktnummer ABIN334425

Kurzübersicht für IDE Antikörper (Internal Region) (ABIN334425)

Target

Alle IDE Antikörper anzeigen
IDE (Insulin-Degrading Enzyme (IDE))

Reaktivität

  • 92
  • 51
  • 48
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Wirt

  • 80
  • 12
  • 3
Ziege

Klonalität

  • 64
  • 31
Polyklonal

Konjugat

  • 37
  • 8
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser IDE Antikörper ist unkonjugiert

Applikation

  • 78
  • 28
  • 26
  • 14
  • 14
  • 13
  • 12
  • 7
  • 5
  • 4
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse

Verified
  • Bindungsspezifität

    • 16
    • 8
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    Verwendungszweck

    Insulysin / Insulinase

    Sequenz

    QQYNFDRDNT E

    Spezifität

    This antibody is expected to recognize all reported isoforms (NP_004960.2, NP_001159418.1, NP_001309722.1, NP_001309723.1, NP_001309724.1, NP_001309726.1). Reported variants represent identical protein: NP_001309725.1, NP_001309724.1

    Kreuzreaktivität

    Hund, Human

    Aufreinigung

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-QQYNFDRDNTE, from the internal region of the protein sequence according to NP_004960.1, NP_001159418.1.

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry: Paraffin embedded Human Lung and Breast. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 110-120 kDa band observed in lysates of cell line K562 (calculated MW of 118 kDa according to NP_004960.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Minimize freezing and thawing.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    IDE (Insulin-Degrading Enzyme (IDE))

    Andere Bezeichnung

    IDE

    Hintergrund

    Insulinase, insulysin, insulin-degrading enzyme , FLJ35968, INSULYSIN

    Gen-ID

    3416

    NCBI Accession

    NP_004960, NP_001159418, NP_001309722, NP_001309723, NP_001309724, NP_001309726

    Pathways

    SARS-CoV-2 Protein Interaktom
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