DRD5 Antikörper (Internal Region)

Produktnummer ABIN334388

Der Ziege Polyklonal anti-DRD5 Antikörper wird verwendet zum Nachweis von DRD5 in Proben von Human. Er wurde validiert für ELISA, IF und FACS.

Kurzübersicht für DRD5 Antikörper (Internal Region) (ABIN334388)

Target: DRD5 (Dopamine Receptor D5 (DRD5))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DRD5 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-DRD5 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: DRD5

Sequenz: NREVDNDEEE GPFD

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NREVDNDEEEGPFD, from the internal region of the protein sequence according to NP_000789.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary testing showed a band at approx 55 kDa in Caco-2 and U2OS cell lysate after 1-2 μg/mL antibody staining (calculated MW of 53.0 kDa according to NP_000789.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membranes of U251 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recomm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu DRD5

Target: DRD5 (Dopamine Receptor D5 (DRD5))

Andere Bezeichnung: DRD5

Hintergrund: DRD5, dopamine receptor D5 , DBDR, DRD1B, DRD1L2, MGC10601 , D1beta dopamine receptor, dopamine receptor D1B

Gen-ID: 1816

NCBI Accession: NP_000789

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity