alpha 2 Antiplasmin Antikörper (Internal Region)

Produktnummer ABIN334343

Der Ziege Polyklonal Anti-alpha 2 Antiplasmin-Antikörper wurde für WB, IHC, ELISA, IF und FACS validiert. Er ist geeignet, alpha 2 Antiplasmin in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für alpha 2 Antiplasmin Antikörper (Internal Region) (ABIN334343)

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser alpha 2 Antiplasmin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-alpha 2 Antiplasmin Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Alpha2-antiplasmin

Sequenz: KDFLQSLKGF PRGDK

Spezifität: This antibody is expected to recognise both reported isoforms (NP_000925.2, NP_001159393.1). Reported variants represent identical protein (NP_000925.2, NP_001159392.1).

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KDFLQSLKGFPRGDK, from the internal region of the protein sequence according to NP_000925.2, NP_001159393.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 70 kDa band observed in lysates of cell line HepG2 (calculated MW of 54.6 kDa according to NP_000925.2). The observed molecular weight is observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at r

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-alpha 2 Antiplasmin Antikörper (ABIN334343)

Abdul, Dekkers, Ariëns, Leebeek, Rijken, Uitte de Willige: "On the localization of the cleavage site in human alpha-2-antiplasmin, involved in the generation of the non-plasminogen binding form." in: Journal of thrombosis and haemostasis : JTH, Vol. 18, Issue 5, pp. 1162-1170, (2020) (PubMed).

Details zu alpha 2 Antiplasmin

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Andere Bezeichnung: SERPINF2

Hintergrund: SERPINF2, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP, ALPHA-2-PI, API, PLI, alpha-2-plasmin inhibitor, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment

Gen-ID: 5345

NCBI Accession: NP_000925, NP_001159393

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones