PTH1R Antikörper (AA 146-169)

Produktnummer ABIN334275

Dieses Anti-PTH1R-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von PTH1R in WB, IHC und IHC (p). Geeignet für Human, Maus, Rind (Kuh), Schwein, Hund, Affe und Pferd.

Kurzübersicht für PTH1R Antikörper (AA 146-169) (ABIN334275)

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Reaktivität: Human, Maus, Rind (Kuh), Schwein, Hund, Affe, Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PTH1R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PTH1R Antikörper

Bindungsspezifität: AA 146-169

Marke: IHC-plus™

Spezifität: Recognizes human PTH/PTHrP receptor, MW 80kD, and the receptor dimer, Mr 160kD. A faint nonspecific band at 110kD is also detected. This antibody does not detect the PTH2 receptor.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Dog, Horse, Pig (100%) Mouse, Rat, Hamster, Panda, Bat, Rabbit (96%) Opossum, Chicken (92%) Xenopus (88%).

Aufreinigung: Protein G purified

Immunogen: Synthetic peptide corresponding to aa146-169 of human PTH/PTHrP Receptor [RRCDRNGSWELVPGHNRTWANYSE]. Species sequence Homology: Mouse & rat (23/24aa). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Dog, Horse, Pig (100%), Mouse, Rat, Hamster, Panda, Bat, Rabbit (96%), Opossum, Chicken (92%), Xenopus (88%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Detects PTH/PTHrP receptor in human kidney, vascular smooth muscle and ovary sections. Receptor was also detected in porcine oocytes. Western Blot: 0.5-2 μg/mL. Detects PTH/PTHrP receptor from RIPA lysates of 3T3-L1 pre-adipose fibroblasts. 3T3-L1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P3109-50. Proteins were visualized using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.6, 0.07 % sodium azide, 30 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PTH1R

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Andere Bezeichnung: PTHR / PTHR1

Hintergrund: Name/Gene ID: PTH1R
Subfamily: Parathyroid hormone
Family: GPCR

Synonyms: PTH1R, Parathyroid hormone 1 receptor, Parathyroid hormone receptor 1, PFE, Pth-related peptide receptor, PTHR, PTH/PTHr receptor, Pth/pthrp receptor, Parathyroid hormone receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR1, PTHrP-R, PTHrP-receptor

Gen-ID: 5745

UniProt: Q03431

Pathways: Regulation of Carbohydrate Metabolic Process