Claudin 4 Antikörper (C-Term)

Produktnummer ABIN334265

Der Maus Monoklonal Anti-Claudin 4-Antikörper wurde für WB, IHC, IF und IHC (p) validiert. Er ist geeignet, Claudin 4 in Proben von Human, Ratte und Hund zu detektieren.

Kurzübersicht für Claudin 4 Antikörper (C-Term) (ABIN334265)

Target: Claudin 4 (CLDN4)

Reaktivität: Human, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Claudin 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Claudin 4 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Recognizes the human ~22kD Claudin 4 protein and does not cross-react with the protein at 55kD. Species cross-reactivity: rat ileum homogenates and canine MDCK cell lysates by Western Blot and Immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by Immunohistochemistry.

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin 4.

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IF (1 - 3 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Details zu Claudin 4

Target: Claudin 4 (CLDN4)

Andere Bezeichnung: CLDN4 / Claudin 4

Hintergrund: Name/Gene ID: CLDN4
Family: Claudin

Synonyms: CLDN4, Claudin-4, CPE-receptor, CPETR, CPETR1, Claudin 4, CPE-R, HCPE-R, WBSCR8, CPER

Gen-ID: 1364

UniProt: O14493

Pathways: Hepatitis C