Actin Antikörper

Produktnummer ABIN331865

Der Maus Monoklonal Anti-Actin-Antikörper wurde für WB, IHC, IHC (p) und IP validiert. Er ist geeignet, Actin in Proben von Human, Ratte, Maus, Huhn und Kaninchen zu detektieren.

Kurzübersicht für Actin Antikörper (ABIN331865)

Target: Actin

Reaktivität: Human, Ratte, Maus, Huhn, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-Actin Antikörper

Spezifität: Reacts with all six known vertebrate isoforms of actin (MW ~42kD). It also reacts with two cytoplasmic actins (beta, gamma) which are highly homologous to one another, but which differ from the muscle actins by about 25 amino acid residues. This antibody is highly recommended for monitoring total protein load on Western blots. Species cross-reactivity: Human, bovine, mouse, canine, porcine, rabbit, rat, chicken, Dictyostelium discoideum, Physarum polycephalum.

Aufreinigung: Protein G purified

Immunogen: Chicken gizzard actin.

Type of Immunogen: Purified protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (0.5 μg/mL), IP, WB (0.5 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Immunohistochemistry and Western Blot. Immunoprecipitation (Denatured verified): 2 μg/mg protein lysate. Use Protein G. Western Blot: 0.5 μg/mL for 2hrs at room temperature. Immunohistochemistry (formalin/paraffin): 1:400 for 30 min. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Positive control: HeLa cells, skeletal muscle. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Chicken

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4.

Konservierungsmittel: Azide free

Details zu Actin

Target: Actin