Actin Antikörper (N-Term)

Produktnummer ABIN331838

Der Maus Monoklonal Anti-Actin-Antikörper wurde für WB, IHC, ELISA, IHC (p) und ICC validiert. Er ist geeignet, Actin in Proben von Human, Ratte, Maus, Huhn, Kaninchen, Meerschweinchen und Schaf zu detektieren.

Kurzübersicht für Actin Antikörper (N-Term) (ABIN331838)

Target: Actin

Reaktivität: Human, Ratte, Maus, Huhn, Kaninchen, Meerschweinchen, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-Actin Antikörper

Bindungsspezifität: AA 50-70, N-Term

Spezifität: Recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms. This clone is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. Labels myotubes and stains myoblasts and fibroblasts. Does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. Species cross-reactivity: All species.

Aufreinigung: Protein A purified

Immunogen: Purified chicken gizzard actin. Epitope: Appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70.

Type of Immunogen: Purified protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC (1 - 20 μg/mL), IHC, IHC-P, WB

Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Immunohistochemistry: 10 μg/mL (paraffin embedded, 4 % formaldehyde, 3 % glutaraldehyde, sodium cacodylate treated sections). Visualized using a donkey anti-mouse IgG (Cy3). Positive staining pattern reflects both membrane and cytoplasmic staining. ELISA: Shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoideum and Physarum polycephalum in ELISA.

Kommentare:

Target Species of Antibody: Chicken

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Actin

Target: Actin