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MLX Antikörper (AA 197-298)

Dieses Anti-MLX-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von MLX in WB, ELISA und ICC. Geeignet für Human.
Produktnummer ABIN328581

Kurzübersicht für MLX Antikörper (AA 197-298) (ABIN328581)

Target

Alle MLX Antikörper anzeigen
MLX (MAX-Like Protein X (MLX))

Reaktivität

  • 60
  • 24
  • 23
  • 11
  • 9
  • 9
  • 8
  • 8
  • 8
  • 5
  • 5
  • 5
  • 4
  • 2
  • 2
  • 1
Human

Wirt

  • 53
  • 5
  • 2
Ziege

Klonalität

  • 57
  • 3
Polyklonal

Konjugat

  • 30
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser MLX Antikörper ist unkonjugiert

Applikation

  • 45
  • 21
  • 13
  • 13
  • 11
  • 7
  • 7
  • 3
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 15
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 197-298

    Spezifität

    Recognizes human MLX. Species sequence homology: 99 % identical to mouse and canine MLX.

    Aufreinigung

    Immunoaffinity purified

    Immunogen

    E. coli-derived recombinant human rhMLX (aa 197-298).

    Type of Immunogen: Recombinant protein

    Isotyp

    IgG
  • Applikationshinweise

    Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: Suitable for use in Western blot, Immunocytochemistry and ELISA. Western blot: 0.1-0.2 μg/mL. The detection limit is ~2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL will detect human MLX in serum stimulated W138 human fetal lung fibroblasts. Fix cells with PBS containing 4 % paraformaldehyde for 20 minutes at RT and block with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. followed by NorthernLights 557 donkey anti-goat lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.3 ng/well.

    Kommentare

    Target Species of Antibody: Human

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitute, 0.5 mL PBS to a concentration of 0.2 mg/mL.

    Konzentration

    Lot specific

    Buffer

    Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 0.5 mL PBS to a concentration of 0.2 mg/mL.
  • Target

    MLX (MAX-Like Protein X (MLX))

    Andere Bezeichnung

    MLX / TCFL4

    Hintergrund

    Name/Gene ID: MLX

    Synonyms: MLX, BHLHd13, BigMax protein, MXD7, MAD7, Max-like bHLHZip protein, TCFL4, MLX, MAX dimerization protein, Protein BigMax, Max-like protein X, Transcription factor-like 4

    Gen-ID

    6945

    UniProt

    Q9UH92
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