MLX Antikörper (AA 197-298)
Kurzübersicht für MLX Antikörper (AA 197-298) (ABIN328581)
Target
Alle MLX Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 197-298
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Spezifität
- Recognizes human MLX. Species sequence homology: 99 % identical to mouse and canine MLX.
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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E. coli-derived recombinant human rhMLX (aa 197-298).
Type of Immunogen: Recombinant protein -
Isotyp
- IgG
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Applikationshinweise
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Western blot, Immunocytochemistry and ELISA. Western blot: 0.1-0.2 μg/mL. The detection limit is ~2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL will detect human MLX in serum stimulated W138 human fetal lung fibroblasts. Fix cells with PBS containing 4 % paraformaldehyde for 20 minutes at RT and block with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. followed by NorthernLights 557 donkey anti-goat lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.3 ng/well. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Reconstitute, 0.5 mL PBS to a concentration of 0.2 mg/mL.
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Konzentration
- Lot specific
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Buffer
- Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 0.5 mL PBS to a concentration of 0.2 mg/mL.
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- MLX (MAX-Like Protein X (MLX))
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Andere Bezeichnung
- MLX / TCFL4
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Hintergrund
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Name/Gene ID: MLX
Synonyms: MLX, BHLHd13, BigMax protein, MXD7, MAD7, Max-like bHLHZip protein, TCFL4, MLX, MAX dimerization protein, Protein BigMax, Max-like protein X, Transcription factor-like 4 -
Gen-ID
- 6945
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UniProt
- Q9UH92
Target
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