CCL13 Antikörper

Produktnummer ABIN328356

Produktdetails anti-CCL13 Antikörper

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CCL13 Antikörper ist unkonjugiert

Applikation: ELISA, Neutralization (Neut), Functional Studies (Func), Flow Cytometry (FACS)

Spezifität: Recognizes human CCL13. In sandwich ELISAs, less than 1 % cross-reactivity was observed with rhMCP-1 and no significant cross-reactivity or interference was observed with rhMCP-2, rhMCP-3, r  mMARC, r  mMCP-5, rmJE, rhEotaxin, rhEotaxin-2, rmEotaxin, rhRANTES or rmRANTES.

Aufreinigung: Protein G purified

Immunogen: E. coli-derived rhCCL13.

Type of Immunogen: Recombinant protein

Klon: 73506

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, Func, Neut

Usage: Neutralization of Bioactivity, Flow Cytometry, ELISA capture. Neutralization of Human CCL13 Bioactivity: The exact concentration of antibody required to neutralize human CCL13 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Flow Cytometry: This antibody was validated for flow cytometry using IL-1 treated A549 cells. For intracellular staining to detect human CCL13, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. ELISA Capture: This antibody can be used as a capture antibody in a human CCL13 ELISA in combination with the biotinylated polyclonal detection antibody. A general protocol is provided on the next page. Using plates coated with 100 μL/well of the capture antibody at 2 μg/mL, in combination with 100 μL/well of the detection antibody, an ELISA for sample volumes of 100 μL can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard starting with 0.5 ng/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Konzentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Details zu CCL13

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Andere Bezeichnung: CCL13 / MCP-4 (CCL13 Produkte)

Hintergrund: Name/Gene ID: CCL13
Family: Intercrine

Synonyms: CCL13, C-C motif chemokine 13, CKb10, MCP-4, MCP4, Monocyte chemotactic protein 4, New CC chemokine 1, Small-inducible cytokine A13, NCC1, CK-beta-10, NCC-1, SCYA13

Gen-ID: 6357

UniProt: Q99616

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Phosphorylierungen bei SARS-CoV-2 Infektion