IL17F Antikörper (AA 31-163)

Produktnummer ABIN327416

Der Maus Monoklonal Anti-IL17F-Antikörper wurde für ELISA und FACS validiert. Er ist geeignet, IL17F in Proben von Human zu detektieren.

Kurzübersicht für IL17F Antikörper (AA 31-163) (ABIN327416)

Target: IL17F (Interleukin 17F (IL17F))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL17F Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS)

Klon: 197301

Produktdetails anti-IL17F Antikörper

Bindungsspezifität: AA 31-163

Spezifität: Recognizes human IL-17F. In direct ELISAs and Western Blots this antibody does not cross-react with rhIL-17A, B, C, D, or E.

Aufreinigung: Protein G purified

Immunogen: E. coli-derived rhIL-17F corresponding to aa 31-163 according to Q96PD4.

Type of Immunogen: Recombinant protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo

Usage: Suitable for use in ELISA and Flow Cytometry. The detection limit is ~25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry (Intracellular): 25 μg/mL. Add 10 μL to 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. For intracellular staining to detect IL-17F, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. This antibody was tested using PMA and Ca2+ ionomycin-treated PBMCs. ELISA (Direct): 0.5-1.0 μg/mL. The detection limit is ~1 ng/well.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 200 μL sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handhabung: Avoid freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

Details zu IL17F

Target: IL17F (Interleukin 17F (IL17F))

Andere Bezeichnung: IL17F

Hintergrund: Name/Gene ID: IL17F

Synonyms: IL17F, CANDF6, Cytokine ML-1, IL-17F, Interleukin-17F, Interleukin 17F, Interleukin-24, ML-1, ML1, Mutant IL-17F

Gen-ID: 112744

UniProt: Q96PD4

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity