IGFBPL1 Antikörper (AA 18-270)
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- Target Alle IGFBPL1 Produkte
- IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))
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Bindungsspezifität
- AA 18-270
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Reaktivität
- Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser IGFBPL1 Antikörper ist unkonjugiert
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Applikation
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Spezifität
- Recognizes mouse IGFBP-L1. (aa 18-270) Cross-reactivity: rhIGFBP-L1:10 % and rmIGFBP-7 less than 1 %. Species sequence homology: Rat 96 %, human 79 %.
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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NS0-derived recombinant rat rmIGFBP-L1.
Type of Immunogen: Recombinant protein - Isotyp
- IgG
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- Applikationshinweise
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Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Western blot, Immunohistochemistry, and Direct ELISA. Western Blot: 0.1-0.2 μg/mL. The detection limit is ~2 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Immunohistochemistry: 10 μg/mL detects IGFBP-L1 in cryosections of E13.5 mouse brain. Tissue was fixed overnight in 4 % PFA+14 % picric acid in PBS at 4? C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 minutes at RT. After. blocking, sections were incubated with primary antibody for 3 hours at RT followed by NorthernLights 557 donkey anti-goat lgG at RT for 1 hour. Between each step, sections were. washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.5 ng/well. - Kommentare
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Target Species of Antibody: Mouse
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Reconstitute, 0.5 mL sterile PBS.
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, 5 % trehalose. Reconstitute, 0.5 mL sterile PBS.
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- Target
- IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))
- Andere Bezeichnung
- IGFBPL1 (IGFBPL1 Produkte)
- Synonyme
- IGFBP-RP4 antikoerper, IGFBPRP4 antikoerper, bA113O24.1 antikoerper, 2810011G06Rik antikoerper, 2810453O06Rik antikoerper, IGFBPL antikoerper, insulin like growth factor binding protein like 1 antikoerper, insulin-like growth factor binding protein-like 1 antikoerper, IGFBPL1 antikoerper, Igfbpl1 antikoerper
- Hintergrund
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Name/Gene ID: IGFBPL1
Synonyms: IGFBPL1, BA113O24.1, IGFBP-RP4, IGFBP-related protein 10, IGFBPRP4 - Gen-ID
- 347252
- UniProt
- Q8WX77
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