IGFBPL1 Antikörper (AA 18-270)

Produktnummer ABIN327188

Der Ziege Polyklonal anti-IGFBPL1 Antikörper wird verwendet zum Nachweis von IGFBPL1 in Proben von Maus. Er wurde validiert für WB, ELISA und IHC.

Kurzübersicht für IGFBPL1 Antikörper (AA 18-270) (ABIN327188)

Target: IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IGFBPL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-IGFBPL1 Antikörper

Bindungsspezifität: AA 18-270

Spezifität: Recognizes mouse IGFBP-L1. (aa 18-270) Cross-reactivity: rhIGFBP-L1:10 % and rmIGFBP-7 less than 1 %. Species sequence homology: Rat 96 %, human 79 %.

Aufreinigung: Immunoaffinity purified

Immunogen: NS0-derived recombinant rat rmIGFBP-L1.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western blot, Immunohistochemistry, and Direct ELISA. Western Blot: 0.1-0.2 μg/mL. The detection limit is ~2 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Immunohistochemistry: 10 μg/mL detects IGFBP-L1 in cryosections of E13.5 mouse brain. Tissue was fixed overnight in 4 % PFA+14 % picric acid in PBS at 4? C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 minutes at RT. After. blocking, sections were incubated with primary antibody for 3 hours at RT followed by NorthernLights 557 donkey anti-goat lgG at RT for 1 hour. Between each step, sections were. washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.5 ng/well.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute, 0.5 mL sterile PBS.

Konzentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitute, 0.5 mL sterile PBS.

Details zu IGFBPL1

Target: IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))

Andere Bezeichnung: IGFBPL1

Hintergrund: Name/Gene ID: IGFBPL1

Synonyms: IGFBPL1, BA113O24.1, IGFBP-RP4, IGFBP-related protein 10, IGFBPRP4

Gen-ID: 347252

UniProt: Q8WX77