Thrombomodulin Antikörper (AA 17-517)

Produktnummer ABIN326112

Dieses Anti-Thrombomodulin-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von Thrombomodulin in WB, IHC, ELISA, FACS, ICC und IHC (fro). Geeignet für Maus.

Kurzübersicht für Thrombomodulin Antikörper (AA 17-517) (ABIN326112)

Target: Thrombomodulin (THBD)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Thrombomodulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 461714

Produktdetails anti-Thrombomodulin Antikörper

Bindungsspezifität: AA 17-517

Spezifität: Recognizes mouse Thrombomodulin.

Aufreinigung: Protein G purified

Immunogen: NS0-derived rmThrombomodulin. (aa 17 - 517).

Type of Immunogen: Recombinant protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo (25 μg/mL), ICC (10 μg/mL), IHC, IHC-Fr, WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow Cytometry, ELISA, Western Blot Immunohistochemistry and Immunocytochemistry. Flow Cytometry: 25 μg/mL, Use 10 μL to label 1-2.5x10^5 bEnd.3 cells in a total reaction volume < 200 μL. ELISA (Direct): 0.5-1.0 μg/mL The detection limit is ~5 ng/well. Western Blot: 1-2 μg/mL Using a colorimetric detection system, the detection limit is ~25 ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold. Immunohistochemistry: 25 μg/mL. Used with frozen sections of normal mouse kidney and heart fixed with 85 mM Na2HPO4, 75 mM KH2P04, 4 % paraformaldehyde, and 14 % (v/v) saturated picric acid,  pH 6.9. Immunocytochemistry: 10 μg/mL. Used with bEnd.3 cells. Cells were fixed with PBS, 4 % paraformaldehyde and blocked with PBS, 10 % normal donkey serum, 0.1 % Triton X-100, 1 % BSA. Between each step, cells were washed with PBS, BSA.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 200 μL sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handhabung: Aviod frequent freeze thaws.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Details zu Thrombomodulin

Target: Thrombomodulin (THBD)

Andere Bezeichnung: THBD / CD141 / Thrombomodulin

Hintergrund: Name/Gene ID: THBD

Synonyms: THBD, BDCA3, CD141 antigen, CD141, AHUS6, Fetomodulin, THPH12, TM, THRM, Thrombomodulin

Gen-ID: 7056

UniProt: P07204