Granulin Antikörper

Produktnummer ABIN325602

Dieses Anti-Granulin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Granulin in WB, IP und ELISA. Geeignet für Human.

Kurzübersicht für Granulin Antikörper (ABIN325602)

Target: Granulin (GRN)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Granulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Produktdetails anti-Granulin Antikörper

Spezifität: Recognizes human GRN. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rmProgranulin.

Aufreinigung: Protein G purified

Immunogen: NS0-derived rhGRN.

Type of Immunogen: Recombinant protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), IP (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1.0 μg/mL, The detection limit for rhGRN is approximately 2 ng/well. Western Blot: 1-2 μg/mL, Using a colorimetric detection system, the detection limit for rhGRN is ~5 ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25 μg/mL to immunoprecipitate rhGRN from conditioned cell culture medium. The recovered rhGRN can be detected in Western Blots using an affinity-purified polyclonal antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 200 μL sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 8.0, 20  mM Tris, 300  mM sodium chloride, 5 % trehalose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Short term: -20°C
Long term: -20°C.

Details zu Granulin

Target: Granulin (GRN)

Andere Bezeichnung: GRN / Progranulin / Granulin

Hintergrund: Name/Gene ID: GRN
Family: Hormone

Synonyms: GRN, Acrogranin, CLN11, Epithelin precursor, GEP, gp88, Granulin, Granulin-epithelin, Granulins, PC cell-derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, Progranulin

Gen-ID: 2896

UniProt: P28799