CCR7 Antikörper

Produktnummer ABIN322907

Der Ratte Monoklonal Anti-CCR7-Antikörper wurde für FACS, IHC, Func und Neut validiert. Er ist geeignet, CCR7 in Proben von Maus zu detektieren.

Kurzübersicht für CCR7 Antikörper (ABIN322907)

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CCR7 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Neutralization (Neut)

Produktdetails anti-CCR7 Antikörper

Spezifität: Recognizes mouse CCR7.

Aufreinigung: Protein G purified

Immunogen: RBC-2H3 cells expressing mCCR7.

Type of Immunogen: Cells

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo, Func, IHC (25 μg/mL), Neut

Usage: Flow cytometry, Immunohistochemistry, Neutralization of Bioactivity. Flow Cytometry-Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: 25 μg/mL with appropriate secondary reagents to detect CCR7 on paraformaldehyde-fixed mouse splenocytes. Neutralization of Murine CCR7 Bioactivity: The exact concentration of antibody required to neutralize mouse CCR7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-mouse CCR7 antibody was determined to be approximately 2-10 μg/mL in the presence of 25 ng/mL of r  mMIP-3, using the BaF/3 mCCR7 cell line in a chemoattraction assay.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CCR7

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Andere Bezeichnung: CCR7

Hintergrund: Name/Gene ID: CCR7
Subfamily: Chemokine
Family: GPCR

Synonyms: CCR7, BLR2, Chemokine (C-C) receptor 7, CD197, C-C CKR-7, CMKBR7, CC chemokine receptor 7, CCR-7, CD197 antigen, CDw197, EBI1, EVI1, MIP-3 beta receptor, C-c chemokine receptor 7, C-C chemokine receptor type 7, CC-CKR-7, Chemokine c-c motif receptor 7

Gen-ID: 1236

UniProt: P32248

Pathways: Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process