Carboxypeptidase A2 Antikörper

Produktnummer ABIN322590

Dieses Anti-Carboxypeptidase A2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Carboxypeptidase A2 in WB, ELISA, IP und Neut. Geeignet für Human.

Kurzübersicht für Carboxypeptidase A2 Antikörper (ABIN322590)

Target: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Carboxypeptidase A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Produktdetails anti-Carboxypeptidase A2 Antikörper

Spezifität: Recognizes the pro form of human CPA2, but not the activated enzyme in Western blots. Species sequence homology: chimpanzee 99.5 % , canine 90 %, rat 87 %, mouse 86 % and chicken 70 %.

Aufreinigung: Protein G purified

Immunogen: NS0-derived recombinant human rhCPA2.

Type of Immunogen: Recombinant protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), IP (25 μg/mL), Neut, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western blot, Neutralization, Direct ELISA and Immunoprecipitation. Western blot:1-2 μg/mL the detection limit is ~10 ng/lane under non-reducing and reducing conditions. will increase sensitivity by 5 to 50 fold. Neutralization: preincubate with the activated enzyme at different molar ratios at room temperature for 30 min. Then assay with N-acetyl-Phe-Thiaphe-OH. 50 % of the enzymatic activity was inhibited at ~1.5 μg/mL (IC50) under conditions in which the enzyme was. present at 0.1 μg/mL and the substrate concentration was 0.1 mM. Considering the. molecular masses of the enzyme (42kD) and the antibody (150kD), IC50 was achieved at. approximately 5:1 Molar ratio of the antibody to the enzyme. Immunoprecipitation: 25 μg/mL. Direct ELISA: 0.5-1.0 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute, 1 mL sterile PBS.

Konzentration: Lot specific

Buffer: Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 1 mL sterile PBS.

Details zu Carboxypeptidase A2

Target: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Andere Bezeichnung: CPA2

Hintergrund: Name/Gene ID: CPA2
Subfamily: Metallopeptidase M14
Family: Exopeptidase

Synonyms: CPA2, Carboxypeptidase A2

Gen-ID: 1358

UniProt: P48052