CD52 Antikörper

Produktnummer ABIN320174

Der Ratte Monoklonal anti-CD52 Antikörper wird verwendet zum Nachweis von CD52 in Proben von Human und Affe. Er wurde validiert für FACS, WB, IP, IHC (fro), CyTox und EIA.

Kurzübersicht für CD52 Antikörper (ABIN320174)

Target: CD52

Reaktivität: Human, Affe

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD52 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Enzyme Immunoassay (EIA)

Klon: YTH34-5

Produktdetails anti-CD52 Antikörper

Spezifität: This antibody reacts with CD52 antigen. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.

Aufreinigung: Affinity Chromatography on Protein A .

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (1/20): Use 10 μL of the suggested working dilution to label 1 x 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections (1/20). Immunohistochemistry on Paraffin Sections (1/20-1/40). Immunohistochemistry on Resin. Positive Control Tissue: Tonsil. ELISA. Western Blotting. Immunoprecipitation. Cytotoxic Assays (50 μg/mL): Use human serum as complement source.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS 0.09 % containing Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD52

Target: CD52

Andere Bezeichnung: CD52 / CAMPATH-1

Hintergrund: CD52 is a small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Synonyms: CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5

Gen-ID: 1043

NCBI Accession: NP_001794

UniProt: P31358