SLA (Haploid Sperm Cell-Specific Antigen) Antikörper

Produktnummer ABIN3201019

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Ratte Monoklonal anti-SLA (Haploid Sperm Cell-Specific Antigen) Antikörper wird verwendet zum Nachweis von SLA (Haploid Sperm Cell-Specific Antigen) in Proben von Maus und Ratte. Er wurde validiert für EM, IHC und WB.

Kurzübersicht für SLA (Haploid Sperm Cell-Specific Antigen) Antikörper (ABIN3201019)

Target: SLA (Haploid Sperm Cell-Specific Antigen)

Reaktivität: Maus, Ratte

Wirt: Ratte

Klonalität: Monoklonal

Applikation: Electron Microscopy (EM), Immunohistochemistry (IHC), Western Blotting (WB)

Klon: TRA54

Produktdetails

Kreuzreaktivität (Details): Not tested in other species.

Produktmerkmale: The antibody was purified from the serum-free culture medium of the hybridoma clone TRA54 by proprietary chromatography processes under mild conditions.
This antibody can be used for immunohistochemical staining of testicular tissues. The hybridoma producing TRA54 was obtained from the immunaization of a rat with cell lysates of adult mouse testes by the group of Prof. Yoshitake Nishimune of Osaka University.

Aufreinigung: Purified

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: 1. Western blotting: ~1 g/mL
2. Immunohistochemical staining
3. Immunoelectron microscopy
Not tested in other applications

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 50 % Glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C

Referenzen

Arrotéia, Joazeiro, Yamada, Tanaka, Nishimune, Pereira: "Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens." in: Journal of andrology, Vol. 25, Issue 6, pp. 914-21, (2004) (PubMed).

Ventelä, Toppari, Parvinen: "Intercellular organelle traffic through cytoplasmic bridges in early spermatids of the rat: mechanisms of haploid gene product sharing." in: Molecular biology of the cell, Vol. 14, Issue 7, pp. 2768-80, (2003) (PubMed).

Pereira, Tanaka, Nagata, Sawada, Mori, Chimelli, Nishimune: "Characterization and expression of a stage specific antigen by monoclonal antibody TRA 54 in testicular germ cells." in: International journal of andrology, Vol. 21, Issue 1, pp. 34-40, (1998) (PubMed).

Antigendetails

Target: SLA (Haploid Sperm Cell-Specific Antigen)

Hintergrund: Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organelles in germ cell cytoplasm from spermatocytes to spermatids. Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis probably due to alternative splicing and/or post-translational modification. The molecular size diversity is due to the difference in glycosilation.