CD151 Antikörper

Produktnummer ABIN319866

Dieses Anti-CD151-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD151 in WB, FACS, IP, IHC (fro) und EIA. Geeignet für Human.

Kurzübersicht für CD151 Antikörper (ABIN319866)

Target: CD151

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD151 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 11G5a

Produktdetails anti-CD151 Antikörper

Spezifität: This antibody recognizes the CD151 cell surface antigen, also known as PETA-3.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL of whole blood. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/100. This product does not require protein digestion pre-treatment but results are enhanced with heat mediated antigen retrieval in 0.01M citrate buffer. Recommended positive control: Tonsil/spleen. Western Blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and coimmunoprecipitation may occur even in quite stringent conditions .

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD151

Target: CD151

Andere Bezeichnung: CD151 / TSPAN24

Hintergrund: CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen.Synonyms: GP27, Membrane glycoprotein SFA-1, PETA-3, PETA3, Platelet-endothelial tetraspan antigen 3, SFA-1, SFA1, Tetraspanin-24

Gen-ID: 977

NCBI Accession: NP_001034579

UniProt: P48509