NOS1 Antikörper (AA 1422-1433)

Produktnummer ABIN3197502

Dieses Anti-NOS1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NOS1 in WB, IP, IF und IHC (p). Geeignet für Human und Ratte.

Kurzübersicht für NOS1 Antikörper (AA 1422-1433) (ABIN3197502)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NOS1 Antikörper

Bindungsspezifität: AA 1422-1433

Sequenz: ESKKDTDEVF SS

Spezifität: This antibody is specific to Nitric-oxide Synthase, Brain (NOS1/nNOS). Predicted

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to the Amino acids 1422-1433 of Human nNOS.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Immunocytochemistry. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: TBS, pH 7.4 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Andere Bezeichnung: NOS1

Hintergrund: Three isoforms of nitric oxide synthase (NOS) have been identified. All are homodimers with subunits of 130-160 kDa. All have binding sites for NADPH, FAD, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain). The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site. Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production. NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO). Neuronal nitric oxide synthase (nNOS, bNOS, cNOS, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane. In response to increased intracellular Ca2+, nNOS interacts with CaM. The Ca2+ CaM complex, in combination with BH4, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm. The dephosphorylation of nNOS by calcineurin initiates the production NO. NO activates guanylyl cyclase (GC) and activates the various cGMP regulated signaling pathways. nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC).Synonyms: Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Nitric Oxide Synthase, bNOS, nNOS

Gen-ID: 4842

NCBI Accession: NP_000611

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction