AGO4 Antikörper

Produktnummer ABIN3197495

Der Kaninchen Polyklonal anti-AGO4 Antikörper wird verwendet zum Nachweis von AGO4 in Proben von Arabidopsis thaliana und Hyacinthus orientalis (Common hyacinth). Er wurde validiert für WB, IP, ICC und Co-IP.

Kurzübersicht für AGO4 Antikörper (ABIN3197495)

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Reaktivität: Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AGO4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Co-Immunoprecipitation ( Co-IP)

Produktdetails anti-AGO4 Antikörper

Verwendungszweck: Anti-AGO4 | Argonaute 4

Kreuzreaktivität (Details): Arabidopsis thaliana, Hyacinthus orientalis
Not reactive in: Brassica oleracea, Fragaria x ananassa, Hordeum vulgare, Poplar sp.,Solanum lycopersicum, Vigna angularis, Zea mays

Homologie: Arabidopsis thaliana

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5

Anwendungsinformationen

Applikationshinweise: 1 : 100 (ICC), 5 μg of antibody per 1 gram of a fresh tissue (IP),1 : 2000-1 : 5000 (WB)

Kommentare:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Note that the AGO4 antibody reacts with the NEB prestained protein marker.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tube.

Details zu AGO4

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Andere Bezeichnung: AGO4

Hintergrund: AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Molekulargewicht: 103 kDa

Gen-ID: 817246

UniProt: Q9ZVD5

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Cellular Glucan Metabolic Process