AGO4 Antikörper

Produktnummer ABIN3197495

Der Kaninchen Polyklonal Anti-AGO4-Antikörper wurde für WB, IP, ICC und Co-IP validiert. Er ist geeignet, AGO4 in Proben von Arabidopsis thaliana und Hyacinthus orientalis (Common hyacinth) zu detektieren.

Kurzübersicht für AGO4 Antikörper (ABIN3197495)

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Reaktivität: Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AGO4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Co-Immunoprecipitation ( Co-IP)

Produktdetails anti-AGO4 Antikörper

Kreuzreaktivität (Details): Not reactive in: Brassica oleracea, Hordeum vulgare, Solanum lycopersicum, Zea mays, Vigna angularis

Homologie: Arabidopsis thaliana

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 103 kDa
Antibody concentration is 0.4 mg/mL in 50% glycerol, PBS, 0.005% thimerosal,0.05% BSA, threfore the antibody sample will not freeze in -20°C.binds enDog (Canine)enous siRNAs, preference for 24nt siRNAs with 5' A.

Aufreinigung: serum

Immunogen: KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5 , TAIR: At2g27040.

Anwendungsinformationen

Applikationshinweise: 1: 4000 (WB), 5 μg of antibody per 1 gram of a fresh tissue (IP), 1: 100 (ICC)

Kommentare:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Note that the AGO4 antibody reacts with the NEB prestained protein marker.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water.

Buffer: Antibody concentration is 0.4 mg/mL in 50 % glycerol, PBS, 0.005 % thimerosal,0.05 % BSA. Please, add 125 µl of deionized water and let it stand in 4 °C overnight to mix. Afterwards warm the antibody sample up to RT and allow theglycerol to dissolve well. Mak

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tubes.

Details zu AGO4

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Andere Bezeichnung: AGO4

Hintergrund: AGI Code: At2g27040
AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Molekulargewicht: 103 kDa

UniProt: Q9ZVD5

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Cellular Glucan Metabolic Process