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AGO4 Antikörper

Der Kaninchen Polyklonal Anti-AGO4-Antikörper wurde für WB, IP, ICC und Co-IP validiert. Er ist geeignet, AGO4 in Proben von Arabidopsis thaliana und Hyacinthus orientalis (Common hyacinth) zu detektieren.
Produktnummer ABIN3197495

Kurzübersicht für AGO4 Antikörper (ABIN3197495)

Target

Alle AGO4 (EIF2C4) Antikörper anzeigen
AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Reaktivität

  • 34
  • 10
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth)

Wirt

  • 31
  • 3
Kaninchen

Klonalität

  • 31
  • 3
Polyklonal

Konjugat

  • 10
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser AGO4 Antikörper ist unkonjugiert

Applikation

  • 21
  • 13
  • 13
  • 10
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Co-Immunoprecipitation ( Co-IP)
  • Kreuzreaktivität (Details)

    Not reactive in: Brassica oleracea, Hordeum vulgare, Solanum lycopersicum, Zea mays, Vigna angularis

    Homologie

    Arabidopsis thaliana

    Produktmerkmale

    Expected / apparent Molecular Weight of the Antigene: 103 kDa
    Antibody concentration is 0.4 mg/mL in 50% glycerol, PBS, 0.005% thimerosal,0.05% BSA, threfore the antibody sample will not freeze in -20°C.binds enDog (Canine)enous siRNAs, preference for 24nt siRNAs with 5' A.

    Aufreinigung

    serum

    Immunogen

    KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5 , TAIR: At2g27040.
  • Applikationshinweise

    1: 4000 (WB), 5 μg of antibody per 1 gram of a fresh tissue (IP), 1: 100 (ICC)

    Kommentare

    AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Note that the AGO4 antibody reacts with the NEB prestained protein marker.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 50 μL of sterile water.

    Buffer

    Antibody concentration is 0.4 mg/mL in 50 % glycerol, PBS, 0.005 % thimerosal,0.05 % BSA. Please, add 125 µl of deionized water and let it stand in 4 °C overnight to mix. Afterwards warm the antibody sample up to RT and allow theglycerol to dissolve well. Mak

    Konservierungsmittel

    Thimerosal (Merthiolate)

    Vorsichtsmaßnahmen

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store at -20°C, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tubes.
  • Target

    AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

    Andere Bezeichnung

    AGO4

    Hintergrund

    AGI Code: At2g27040
    AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

    Molekulargewicht

    103 kDa

    UniProt

    Q9ZVD5

    Pathways

    Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Cellular Glucan Metabolic Process
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