Der Kaninchen Polyklonal anti-Listeria Monocytogenes Antikörper wird verwendet zum Nachweis von Listeria Monocytogenes in Proben von Listeria monocytogenes. Er wurde validiert für EIA und IF.
Reaktivität: Listeria monocytogenes
ELISA, IFA
Wirt: Kaninchen
Polyclonal
FITC
Applikationshinweise
Immunofluorscence and ELISA. Enzyme amplification following reaction with FITC conjugate can also be accomplishedutilizing enzyme-antibody conjugates specific to FITC. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
4-5 mg/mL (OD280 nm, E0.1% = 1.4)
Buffer
0.01 M PBS pH 7.2 with 0.09 % Sodium Azide as preservative and 10 mg/mL BSA as stabilizer.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
Listeria Monocytogenes
Substanzklasse
Bacteria
Hintergrund
The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.