This antibody recognises erythrocytes of normal Rh types but does not recognise Rhnull erythrocytes. It reacts with 31 kDa and 35-51 kDa proteins which were designated CD240DCE at the 7th leucocyte typing workshop.
RHCE
Reaktivität: Human
Wirt: Kaninchen
Polyclonal
Alexa Fluor 680
Applikationshinweise
Immunoprecipitation. Flow Cytometry: 10l of the suggested working dilution to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0mg/mL
Buffer
Tris buffered glycine, pH 7.7 containing 0.09 % Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene which encodes both the RhC and RhE antigens on a single polypeptide and a second gene which encodes the RhD protein. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. A mutation in this gene results in amorph-type Rh-null disease. There are thirteen named isoforms.Synonyms: Blood group Rh(CE) polypeptide, Rh polypeptide 1, Rh30A, RhIXB, Rhesus C/E antigens