EIF4G1 Antikörper (Ser1232)

Produktnummer ABIN319387

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EIF4G1 in WB, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für EIF4G1 Antikörper (Ser1232) (ABIN319387)

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF4G1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-EIF4G1 Antikörper

Bindungsspezifität: Ser1232

Spezifität: This antibody detects endogenous levels of total eLF4G protein.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human eIF4G around the phosphorylation site of Serine 1232 (P-V-Sp-P-L).

Anwendungsinformationen

Applikationshinweise: Western Blot (1/500-1/1000). Immunohistochemistry (1/50-1/100). Immunofluorescence (1/100-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at-20 °C.

Details zu EIF4G1

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Andere Bezeichnung: EIF4G1 / EIF4F

Hintergrund: EIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.Synonyms: EIF4G, EIF4GI, Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220

Gen-ID: 1981

NCBI Accession: NP_886553

UniProt: Q04637