Cofilin Antikörper (Tyr88)

Produktnummer ABIN319381

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Cofilin in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für Cofilin Antikörper (Tyr88) (ABIN319381)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cofilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Cofilin Antikörper

Bindungsspezifität: Tyr88

Spezifität: This antibody detects endogenous levels of total Cofilin-1/2 protein.

Kreuzreaktivität (Details): Also reacts to: Cofilin 2

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Cofilin1/Cofilin2 around the phosphorylation site of Tyrosine 88 (A-T-Yp-E-T).

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at-20 °C.

Details zu Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Andere Bezeichnung: Cofilin-1

Hintergrund: Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors such as opsonized zymosan. Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18

Gen-ID: 1072

NCBI Accession: NP_005498

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events