Dynamin 1 Antikörper (pSer774)

Produktnummer ABIN319098

Dieses Anti-Dynamin 1-Antikörper ist ein Schaf Polyklonal-Antikörper zur Detektion von Dynamin 1 in WB, IF und IHC (fro). Geeignet für Human, Maus, Ratte, Hund, Rind (Kuh), Huhn und Affe.

Kurzübersicht für Dynamin 1 Antikörper (pSer774) (ABIN319098)

Target: Dynamin 1 (DNM1)

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Huhn, Affe

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Dynamin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Dynamin 1 Antikörper

Bindungsspezifität: pSer774

Spezifität: This antibody is specific for Dynamin when phosphorylated at Serine 774.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Monkey, Bovine, Chicken, Mouse, Dog.
Species reactivity (tested):Rat.

Aufreinigung: Affinity Chromatography.

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within Dynamin which includes phosphorylated Ser774. Genename: DNM

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunofluorescence. Immunohistochemistry on Frozen Sections. Western Blot (1/1000): This antibody detects a band of approximately 95 kDa in Rathippocampus lysates. For the detection of Phosphoproteins, Serine Phosphatase inhibitors such as 10 mMSodium Fluoride should be added to the sample buffer. Milk or other casein-basedblocking solutions are not recommended as casein is a Phosphoprotein and its use canresult in high background.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % BSA and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Dynamin 1

Target: Dynamin 1 (DNM1)

Andere Bezeichnung: Dynamin-1

Hintergrund: Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.Synonyms: DNM, DNM1

Gen-ID: 140694

NCBI Accession: NP_542420

UniProt: P21575

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling