CD84 Antikörper (Internal Region)

Produktnummer ABIN3187786

Dieses Anti-CD84-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CD84 in WB, ELISA und IHC (p). Geeignet für Human und Maus. Unabhängig validiert für die Verwendung in Immunohistochemistry.

Kurzübersicht für CD84 Antikörper (Internal Region) (ABIN3187786)

Target: CD84

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD84 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CD84 Antikörper

Bindungsspezifität: Internal Region

Spezifität: CD84 Polyclonal Antibody detects endogenous levels of CD84 protein.

Produktmerkmale: Rabbit Polyclonal to CD84.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CD84.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC-P 1:100-300, ELISA 1:20000,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IHC)

Validierung #104537 (Immunohistochemistry)

by: TRON

No.: #104537

Datum: 07.12.2023

Antigen: CD84

Chargennummer: 15601601

Validierte Anwendung: Immunohistochemistry

Positivkontrolle:

FFPE human tonsil

Bewertung:

Passed. The CD84 antibody ABIN3187786 specifically stains cells within the germinal zone in human tonsil.

Validierungsbilder

IHC staining of human tonsil with anti-CD84 antibody ABIN3187786 (1:100) and counterstain with hematoxylin.

IHC staining of human tonsil with anti-PD-1 antibody ABIN3187786 (1:200) and counterstain with hematoxylin.

Protokoll

Primärantikörper: ABIN3187786

Sekundärantikörper: HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122)

Full Protocol:

• Dewax and rehydrate tissue sections on glass slides in a descending xylene and graded alcohol series:
• • xylene 2x for 5 min.
  • 100 % (v/v) ethanol 2x for 5 min.
  • 96 % (v/v) ethanol 2x for 5 min.
  • 70 % (v/v) ethanol 2x for 5 min.
• Wash slides 2x 5 min with ddH2O.
• Cook slides for 30 min at 95 °C in citrate buffer pH 6.
• Wash slides 2x 3 min with ddH2O.
• Incubate slides in 0.3 % (v/v) H2O2 in PBS for 15 min to quench endogenous peroxidase.
• Wash slides 3x 3 min in 1X PBS.
• Block slides in 10 % goat serum in PBS for 30 min at RT.
• Incubate slides with primary rabbit anti-CD84 antibody (antibodies-online, ABIN3187786, lot 15601601) diluted 1:100 and 1:200 in 10 % goat serum in PBS ON at 4 °C in a humidified chamber.
• Wash slides 3x 3 min in 1X PBS.
• Incubate slides with secondary HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122) ready-to-use in 10 % goat serum in PBS for 30 min at RT in a humidified chamber.
• Wash slides 3x 3 min in 1X PBS.
• HRP development.
• Counterstain slides with hematoxylin solution (Carl Roth, T865.2).
• Wash slides for 15 min under running tap water.
• Dehydrate sections in an ascending alcohol-xylene series:
• • 70% ethanol 2x for 10 sec.
  • 96% ethanol 2x for 10 sec.
  • 100% ethanol 2x for 10 sec.
  • xylene 2x for 10 sec.
• Mount sections with Entellan new mounting medium (Sigma-Aldrich, 1008690500).
• Dry sections.
• Image acquisition on an Aperio CS2 Digital Pathology Slide Scanner (Leica), Magnification 40x.

Anmerkungen:

• Staining with ABIN3187786 overnight at 1:100 and 1:200 antibody dilution the signal in human tonsil was sharp and localized in the cells within the germinal zone (as expected). Modifying ABIN3187786 incubation conditions to 1 h at RT, the signal in human tonsil was still sharp and localized in the cells within the germinal zone.

• However, in mouse spleen, the signal was almost absent and very weak. With the modified incubation conditions, the signal in mouse spleen was again almost absent and very weak. With either staining protocol the signal in mouse lung was sporadic and its quality does not allow the possibility of non-specific staining to be excluded.

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C.

Details zu CD84

Target: CD84

Andere Bezeichnung: CD84

Molekulargewicht: 39 kDa

Gen-ID: 8832

UniProt: Q9UIB8