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Actin Antikörper

Dieses Anti-Actin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Actin in WB. Geeignet für Pflanzen. Unabhängig validiert für die Verwendung in Western Blotting. Dieses Primary Antibody wurde in 1 Publikation zitiert.
Produktnummer ABIN3181106

Kurzübersicht für Actin Antikörper (ABIN3181106)

Target

Alle Actin Antikörper anzeigen
Actin

Reaktivität

  • 103
  • 79
  • 75
  • 27
  • 22
  • 21
  • 11
  • 11
  • 6
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Pflanzen

Wirt

  • 88
  • 42
  • 3
  • 1
  • 1
  • 1
Maus

Klonalität

  • 80
  • 56
Monoklonal

Konjugat

  • 79
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Dieser Actin Antikörper ist unkonjugiert

Applikation

  • 108
  • 42
  • 32
  • 29
  • 28
  • 27
  • 27
  • 22
  • 18
  • 16
  • 15
  • 3
  • 1
Western Blotting (WB)

Klon

Q30
  • Spezifität

    The antibody detects endogenous plant actin protein.

    Produktmerkmale

    Mouse Monoclonal to Plant actin.

    Aufreinigung

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

    Isotyp

    IgG1
  • Applikationshinweise

    WB 1:2000-5000

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Validierung #102343 (Western Blotting)
    'Independent Validation' Siegel
    by
    Department of Horticultural genetics and Biotechnology, Mediterranean Agronomic Institute of Chania
    No.
    #102343
    Datum
    19.12.2017
    Antigen
    Actin
    Chargennummer
    B3401
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    protein extraction from olive root tissue and tomato flower tissue
    Negativkontrolle
    Bewertung

    Passed. ABIN3181106 specifically recognizes the actin in both tomato flower and olive roots samples, providing a clear band at the detection with the secondary one.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN3181106
    Sekundärantikörper
    HRP conjugated rabbit anti-mouse IgG (Thermo Scientific, PA1-28902, OK1782904)
    Full Protocol
    • Lyse protein extraction from 500mg olive root tissue and 80mg from tomato flower tissue in 240µl cold lysis buffer (1M Tris-Cl pH6.8, 10% SDS, 14.3M β-Merc, 0.5N EDTA, PMSF 0.5mM and 100µl proteinase inhibitor (Sigma-Aldrich, P9599-1ML)).
    • Determine total protein content of the lysates using protein assay (Bradford curve).
    • Denature 0.7μg/μl of total protein for 5min at 95°C 1:4 (buffer:protein) in Laemmli SDS sample buffer (Tris-Cl pH6.8, 2% SDS, 10% glycerol, 1%β-mercaptoethanol, 12.5mM EDTA, 0.02% bromophenol blue) and subsequently separate them on a freshly cast denaturing SDS-PAGE gel (30% Bis-Acrylamide, 1.5M Tris pH8.8, 10% SDS, 10% APS and TEMED) in an Bio-Rad Mini-PROTEAN 3 cell systemfor 2h at 100V, 200mA.
    • Transfer proteins onto an Immobilon-P membrane (Millipore, IPVPH0010, K5CA2260G) with a Western blotting system for 1h at 100V.
    • Block the membrane with 5% non-fat milk in 1x TBST for 1h at RT.
    • Incubate membrane with anti-Actin antibody (antibodies-online, ABIN3181106, lot B3401) diluted 1:2500 (Flower samples) or 1:1000 (root samples) in 5% non-fat milk in 1x TBST ON at 4°C.
    • Wash membrane 3x for 5min with 1x TBST.
    • Incubate membrane with secondary HRP conjugated rabbit anti-mouse IgG (Thermo Scientific, PA1-28902, OK1782904) diluted 1: 5000 (flower samples) and 1:2000 (root samples) in 5% non-fat milk in 1x TBSTfor 1h at RT.
    • Wash membrane 3x for 5min with 1X TBST.
    • Reveal protein bands using super signal west pico (Thermo-Scientific, 34080, QF218483) on a Super RX Fuji Medical X-RAY (18cm x 24cm) film.
    Anmerkungen

    The actin antibody ABIN3181106 reveals the actin protein of the expected molecular weight of antigen in lysates of tomato flower and olive root samples.

  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freeze/thaw cycles.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store at -20°C.
  • Hoffmann, Dietrich, Herrmann, Schacht, Albrecht, Methner: "Dimethyl Fumarate Induces Glutathione Recycling by Upregulation of Glutathione Reductase." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 6093903, (2017) (PubMed).

  • Target

    Actin
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