GRK1 Antikörper

Produktnummer ABIN317886

Der Kaninchen Polyklonal anti-GRK1 Antikörper (ABIN317886) detektiert spezifisch GRK1 in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für GRK1 Antikörper (ABIN317886)

Target: GRK1 (G Protein-Coupled Receptor Kinase 1 (GRK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GRK1 Antikörper

Spezifität: GRK1 antibody detects endogenous levels of GRK1 protein.(region surrounding Ala17)

Aufreinigung: Affinity chromatography

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GRK1

Target: GRK1 (G Protein-Coupled Receptor Kinase 1 (GRK1))

Andere Bezeichnung: Rhodopsin Kinase

Hintergrund: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messengerregulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1α and β), two forms of β-adrenergic receptor kinase: GRK 2 (βARK, βARK1) and GRK 3 (βARK2), IT-11 (GRK 4), GRK 5, GRK 6, and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.Synonyms: G protein-coupled receptor kinase 1, GRK1, RHOK

Molekulargewicht: approx. 63 kDa

Gen-ID: 6011

NCBI Accession: NP_002920

UniProt: Q15835

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction