GRB14 Antikörper

Produktnummer ABIN317840

Der Kaninchen Polyklonal anti-GRB14 Antikörper wird verwendet zum Nachweis von GRB14 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC (p).

Kurzübersicht für GRB14 Antikörper (ABIN317840)

Target: GRB14 (Growth Factor Receptor-Bound Protein 14 (GRB14))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRB14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GRB14 Antikörper

Spezifität: GRB14 antibody detects endogenous levels of GRB14 protein.(region surrounding Asp123)

Aufreinigung: Affinity chromatography

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GRB14

Target: GRB14 (Growth Factor Receptor-Bound Protein 14 (GRB14))

Andere Bezeichnung: GRB14

Hintergrund: Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific phosphotyrosine-containing receptor sequences. GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP and a proline-rich N terminus. A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7. GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment, but appears to bind to the EGF receptor poorly. An additional member of the GRB7 family, designated GRB14, contains a pleckstrin homology domain in its central region and a carboxy terminal SH2 domain.Synonyms: GRB14 adapter protein, Growth factor receptor-bound protein 14

Molekulargewicht: approx. 61 kDa

Gen-ID: 2888

NCBI Accession: NP_004481

UniProt: Q14449