RNF144A Antikörper

Produktnummer ABIN317831

Der Kaninchen Polyklonal Anti-RNF144A-Antikörper wurde für WB und EIA validiert. Er ist geeignet, RNF144A in Proben von Human und Maus zu detektieren.

Kurzübersicht für RNF144A Antikörper (ABIN317831)

Target: RNF144A (Ring Finger Protein 144A (RNF144A))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNF144A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-RNF144A Antikörper

Spezifität: UBCE7IP4 antibody detects endogenous levels of UBCE7IP4 protein.(region surrounding Arg184)

Aufreinigung: Affinity chromatography

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu RNF144A

Target: RNF144A (Ring Finger Protein 144A (RNF144A))

Andere Bezeichnung: RNF144A / UBCE7IP4

Hintergrund: Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction.The three classes of enzymes involved in ubiquitination are the ubiquitinactivating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). UBCE7IP4 (Ubiquitin-conjugating enzyme 7- interacting protein 4), also known as RNF144A (RING finger protein 144A), KIAA0161 or RNF144, is a 292 amino acid single-pass membrane protein that contains one RING-type zinc finger and two IBR-type zinc fingers. Functioning as an E3 ubiquitin-protein ligase, UBCE7IP4 accepts ubiquitin (in the form of a thioester) from E2 ubiquitin-conjugating enzymes, such as UBC8 and UBCH7, and transfers that ubiquitin residue to target substrates. Via its RING finger, UBCE7IP4 may play a role in protein-DNA and protein-protein interactions throughout the cell.Synonyms: KIAA0161, Probable E3 ubiquitin-protein ligase RNF144A, RING finger protein 144A, RNF144, UbcM4-interacting protein 4, Ubiquitin-conjugating enzyme 7-interacting protein 4, Uip4

Molekulargewicht: approx. 30 kDa

Gen-ID: 9781

NCBI Accession: NP_055561

UniProt: P50876