ARFGEF2 Antikörper

Produktnummer ABIN317822

Produktdetails anti-ARFGEF2 Antikörper

Target: ARFGEF2 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 2 (Brefeldin A-Inhibited) (ARFGEF2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARFGEF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: BIG2 antibody detects endogenous levels of BIG2 protein.(region surrounding Leu1527)

Aufreinigung: Affinity chromatography

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ARFGEF2

Target: ARFGEF2 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 2 (Brefeldin A-Inhibited) (ARFGEF2))

Andere Bezeichnung: ARFGEF2 (ARFGEF2 Produkte)

Synonyme: AI463430 antikoerper, AW493672 antikoerper, BIG2 antikoerper, E230011G24Rik antikoerper, Big2 antikoerper, PVNH2 antikoerper, dJ1164I10.1 antikoerper, ADP ribosylation factor guanine nucleotide exchange factor 2 antikoerper, ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) antikoerper, ARFGEF2 antikoerper, Arfgef2 antikoerper

Hintergrund: Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs), a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking. GEPs comprise two major families, large GEPs that are inhibited by brefeldin A (BFA), a protein that effects golgi structure, and a group of smaller GEPs that are insenstive to BFA. Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs of approximately 200 kDa, designated BIG1 and BIG2. Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity. In vivo, BIG1 and BIG2 exist in macromolecular complexes that move between the golgi membranes and cytosol. BIG2 associates with PKA regulatory subunits, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways.Synonyms: ARFGEP2, BIG2, Brefeldin A-inhibited GEP2, Brefeldin A-inhibited guanine nucleotide-exchange protein 2

Molekulargewicht: approx. 210 kDa

Gen-ID: 10564

NCBI Accession: NP_006411

UniProt: Q9Y6D5