JIP3 Antikörper

Produktnummer ABIN317721

Der Kaninchen Polyklonal anti-JIP3 Antikörper (ABIN317721) detektiert spezifisch JIP3 in WB, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für JIP3 Antikörper (ABIN317721)

Target: JIP3 (MAPK8IP3) (Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JIP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-JIP3 Antikörper

Spezifität: This antibody detects endogenous levels of JIP-3 protein.(region surrounding Gln649)

Kreuzreaktivität (Details): Species reactivity (tested):Human and Mouse.

Aufreinigung: Affinity Chromatography using epitope-specific Immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/20000approx. 1/40000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200. Immunofluorescence: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu JIP3

Target: JIP3 (MAPK8IP3) (Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3))

Andere Bezeichnung: JIP-3

Hintergrund: C-Jun NH2-terminal kinases (JNKs) are distant members of the MAP kinase family. JNK1 is activated by dual phosphorylation at a Thr-Pro-Tyr motif in response to ultraviolet (UV) light, and it functions to phosphorylate c-Jun at amino terminal serine regulatory sites Ser 63 and Ser 73, resulting in transcriptional activation. Two additional JNK family members, JNK2 and JNK3, have been identified. JIP-1 (for JNK interacting protein-1) has been identified as a cytoplasmic inhibitor of JNK that retains JNK in the cytoplasm, thereby inhibiting JNK-regulated gene expression. Evidence suggests that JNK1 and JNK2 bind to JIP-1 with greater affinity than to ATF-2 and c-Jun, which are targets of the JNK signaling pathway. JIP-1 contains an amino terminal JNK binding domain and a carboxy terminal SH3 domain. ATF-2 and c-Jun also contain the JNK binding domain and are thought to compete with JIP-1 for JNK binding. Multiple splice variants of JIP-1, including JIP-1b, JIP-1c (also designated islet-brain 1 or IB-1), JIP-2a, JIP-2b and JIP-3, have been identified in brain.Synonyms: C-jun-amino-terminal kinase-interacting protein 3, JIP3, JNK MAP kinase scaffold protein 3, JNK-interacting protein 3, MAPK8IP3, Mitogen-activated protein kinase 8-interacting protein 3

Gen-ID: 23162

NCBI Accession: NP_001035529

UniProt: Q9UPT6