STEAP1 Antikörper (N-Term)

Produktnummer ABIN317568

Der Maus Monoklonal anti-STEAP1 Antikörper (Klon J2D2) (ABIN317568) detektiert spezifisch STEAP1 in WB, IF, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für STEAP1 Antikörper (N-Term) (ABIN317568)

Target: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser STEAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: J2D2

Produktdetails anti-STEAP1 Antikörper

Bindungsspezifität: AA 1-70, N-Term

Spezifität: The antibody recognizes STEAP1.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Rat.

Produktmerkmale: Synonyms: STEAP-1, Metalloreductase STEAP1, PRSS24, Six-transmembrane epithelial antigen ofprostate 1

Aufreinigung: Protein-G affinity chromatography

Immunogen: Recombinant human STEAP1 (1-70 aa) purified from E. coli

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot (cell lysates of LNcap (30 μg) and Rat testis (30 μg) were resolved bySDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1: 500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRPand an ECL detection system). Immunofluorescence (1: 100 - 1: 300). Immunohistochemsitry on paraffin sections (1: 50 - 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu STEAP1

Target: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Andere Bezeichnung: STEAP / STEAP1

Hintergrund: Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy. NCBI Accession No: NP_036581Synonyms: Metalloreductase STEAP1, PRSS24, STEAP-1, Six-transmembrane epithelial antigen of prostate 1

Gen-ID: 26872

UniProt: Q9UHE8

Pathways: Transition Metal Ion Homeostasis