APP Antikörper (AA 18-289)

Produktnummer ABIN317496

Der Maus Monoklonal anti-APP Antikörper (Klon J4H9) (ABIN317496) detektiert spezifisch APP in WB, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für APP Antikörper (AA 18-289) (ABIN317496)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser APP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: J4H9

Produktdetails anti-APP Antikörper

Bindungsspezifität: AA 18-289

Spezifität: The antibody recognizes APP.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Produktmerkmale: Synonyms: Alzheimer disease amyloid protein, Amyloid Precursor Protein, ABPP, APPI, PreA4, Cerebralvascular amyloid peptide, CVAP, Protease nexin-II

Aufreinigung: Protein-G affinity chromatography

Immunogen: Recombinant human APP (18-289aa) purified from E. coli

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot (1/500-1/1,000). Immunohistochemistry on Paraffin Sections (2.5 μg/mL). This APP antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Andere Bezeichnung: Amyloid beta A4 Protein / APP

Hintergrund: Amyloid precursor protein (APP) is the precursor molecule whose proteolysis generates amyloid beta (Aβ), a 39- to 42- amino acid peptide and this amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's diseases patients. APP is an integral membrane protein that is phosphorylated in the cytoplasmic and extracellular domains. It has been reported that cell-surface APP plays a role in neurite extension of primary cultured hippocampal neurons. The large extracellular domain of APP is also reported to bind extracellular matrix molecules such as heparin, laminin, and collagen, which can mediate cell adhesion and neurite outgrowth. Abnormal regulation of the metabolism of APP may contribute to the deposition of plaques.Synonyms: ABPP, APPI, Alzheimer disease amyloid protein, Amyloid Precursor Protein, CVAP, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II

Gen-ID: 351

UniProt: P05067

Pathways: Caspase Kaskade in der Apoptose, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour