This antibody recognises CD16, a 50-65 kDa cell surface molecule. Studies show that the DJ130c antibody recognises an epitope in the first membrane-distal domain of CD16 and recognises both CD16A and CD16B.
Produktmerkmale
Synonyms: IgG Fc receptor III-2, Fc-gamma RIII-alpha, FcR-10, CD16a, FCGR3A, FCG3, IGFR3, Fc-gammaRIIIa, FcRIIIa, Fc-gamma RIII, Low affinity immunoglobulin gamma Fc region receptor III-A
Aufreinigung
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 1.0 mL distilled water
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch.
Haltbarkeit
12 months
Target
CD16
Andere Bezeichnung
CD16
Hintergrund
CD16 is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes. In addition, CD16B exists as two allelic variants NA1 and NA2. DJ130c recognises all polymorphonuclear cells irrespective on their NA phenotype.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A