STN8 Kinase (AA 425-438) Antikörper

Produktnummer ABIN3155736

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch STN8 Kinase in WB. Es zeigt Reaktivität gegenüber Proben von Arabidopsis thaliana und Triticum aestivum.Es wurde in 1 Publikation zitiert.

Kurzübersicht für STN8 Kinase (AA 425-438) Antikörper (ABIN3155736)

Target: STN8 Kinase

Reaktivität: Arabidopsis thaliana, Triticum aestivum

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails

Bindungsspezifität: AA 425-438

Verwendungszweck: Anti-STN8 | Serine/threonine-protein kinase STN8 (chloroplastic)

Kreuzreaktivität (Details): Arabidopsis thaliana, Triticum aestivum
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Oryza sativa

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conugated synthetic peptide (amino acids 425-438) specific for Arabidopsis thaliana STN8 serine/threonine proteinkinase

Anwendungsinformationen

Applikationshinweise: 1 : 2000 (WB)

Kommentare:

For best results with this antibody sample buffer needs to contain 6 M urea (138mM TrisHCl pH 6.8, 6M urea, 22.2% Glycerol, 4.3% SDS).STN8 is a nuclear encoded protein which is localized in chloroplast, hence posses a chloroplastic target peptides (cTP) at the beginning of the amino acid sequence which is cut off. Accroding to TargetP (program that predicts the length of the cTP:s) the length of cTP for Stn8 is 49 amino acids. STN8 portein mobility can be also affected by urea present in the gel.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen

Woeber: "Relationship between thyroid stimulating hormone and thyroid stimulating immunoglobulin in Graves' hyperthyroidism." in: Journal of endocrinological investigation, Vol. 34, Issue 3, pp. 222-4, (2011) (PubMed).

Antigendetails

Target: STN8 Kinase

Hintergrund:

Background: Serine/threonine protein kinase (EC=2.7.11.1), known as STN8 in Arabidopsis, and its Stl1 homologue in Chlamydomonas, are STN7/Stt7-like proteins that are not required for state transitions. The Arabidopsis mutants deficient in stn8 gene demonstrated highly reduced levels of the phosphorylated proteins of the photosystem II (Bonardi et al., 2005 Varionen et al., 2005). Alternative name: Protein STATE TRANSITION 8.

Additional information: An extract from STN8 mutant needs to be used in pararel to determine specific band of STN8 protein on a western blot

Molekulargewicht: 54.9 kDa or 46 kDa on 6 M urea gel

Gen-ID: 831741

UniProt: Q9LZV4