VDAC1 Antikörper

Produktnummer ABIN3155731

Dieses Anti-VDAC1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von VDAC1 in WB, IL und BN PAGE. Geeignet für Arabidopsis thaliana, Moss, Papaver und Spinach. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für VDAC1 Antikörper (ABIN3155731)

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Reaktivität: Arabidopsis thaliana, Moss, Papaver, Spinach

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VDAC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunolocalization (IL), Blue-native PAGE (BN PAGE)

Produktdetails anti-VDAC1 Antikörper

Kreuzreaktivität (Details): No cross-reactivity with: Chlamydomonas reinhardtii, Glycine max, Zea mays, diatoms

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 29 kDa (for Arabidopsis thaliana)

Aufreinigung: serum

Immunogen: KLH-conjugated peptide conserved in all known higher plant VDAC1 proteins including Arabidopsis thaliana with the locus name: At3g01280

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 5000 on 2-30 µg of protein/lane with standard ECL (WB), 1 : 500 (IL).
Cellular [compartment marker] of mitochondrial outer membrane

Kommentare:

Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are available hereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 µL of sterile water.

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen für anti-VDAC1 Antikörper (ABIN3155731)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Barreto, Okura, Neshich, Maia, Arruda: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).

Zhou, Zhao, Yang, Chen, Liu, Jin, Chen, Li, Deng, Schumaker, Guo: "Ubiquitin-specific protease16 modulates salt tolerance in Arabidopsis by regulating Na(+)/H(+) antiport activity and serine hydroxymethyltransferase stability." in: The Plant cell, Vol. 24, Issue 12, pp. 5106-22, (2013) (PubMed).

Takahashi, Teranishi, Ishida, Kawasaki, Takeuchi, Yamaya, Watanabe, Makino, Hidema: "CPD photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA." in: The Plant journal : for cell and molecular biology, (2011) (PubMed).

Ito, Batth, Petzold, Redding-Johanson, Mukhopadhyay, Verboom, Meyer, Millar, Heazlewood: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).

Details zu VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Andere Bezeichnung: VDAC1

Hintergrund: AGI Code: At3g01280
VDAC1 protein (called also Synonymes: At3g01280, outer mitochondrial membrane protein porin 1, T22N4_9, T22N4.9, VDAC 1, Voltage-dependent anion-selective channel protein 1, voltage-gated ion-selective channel) forms a channel through the cell membrane for diffusion of small hydrophilic molecules. Evolutionary origin of VDAC protein is not clear and their structure and properties are quite different making those proteins only conceptually like porins (Clausen et al. 2004).

Molekulargewicht: 29 kDa (for Arabidopsis thaliana)

UniProt: Q9SRH5