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VDAC1 Antikörper

Dieses Anti-VDAC1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von VDAC1 in WB, IL und BN PAGE. Geeignet für Arabidopsis thaliana, Moss, Papaver und Spinach. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.
Produktnummer ABIN3155731

Kurzübersicht für VDAC1 Antikörper (ABIN3155731)

Target

Alle VDAC1 Antikörper anzeigen
VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Reaktivität

  • 88
  • 43
  • 31
  • 6
  • 5
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
Arabidopsis thaliana, Moss, Papaver, Spinach

Wirt

  • 72
  • 27
  • 2
Kaninchen

Klonalität

  • 61
  • 40
Polyklonal

Konjugat

  • 60
  • 6
  • 5
  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser VDAC1 Antikörper ist unkonjugiert

Applikation

  • 87
  • 54
  • 40
  • 22
  • 19
  • 13
  • 13
  • 9
  • 7
  • 7
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunolocalization (IL), Blue-native PAGE (BN PAGE)
  • Kreuzreaktivität (Details)

    No cross-reactivity with: Chlamydomonas reinhardtii, Glycine max, Zea mays, diatoms

    Produktmerkmale

    Expected / apparent Molecular Weight of the Antigene: 29 kDa (for Arabidopsis thaliana)

    Aufreinigung

    serum

    Immunogen

    KLH-conjugated peptide conserved in all known higher plant VDAC1 proteins including Arabidopsis thaliana with the locus name: At3g01280
  • Applikationshinweise

    Recommended Dilution: 1 : 5000 on 2-30 µg of protein/lane with standard ECL (WB), 1 : 500 (IL).
    Cellular [compartment marker] of mitochondrial outer membrane

    Kommentare

    Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are available hereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 200 µL of sterile water.

    Buffer

    PBS pH 7.4

    Handhabung

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Lagerung

    -20 °C
  • Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

    Barreto, Okura, Neshich, Maia, Arruda: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).

    Zhou, Zhao, Yang, Chen, Liu, Jin, Chen, Li, Deng, Schumaker, Guo: "Ubiquitin-specific protease16 modulates salt tolerance in Arabidopsis by regulating Na(+)/H(+) antiport activity and serine hydroxymethyltransferase stability." in: The Plant cell, Vol. 24, Issue 12, pp. 5106-22, (2013) (PubMed).

    Takahashi, Teranishi, Ishida, Kawasaki, Takeuchi, Yamaya, Watanabe, Makino, Hidema: "CPD photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA." in: The Plant journal : for cell and molecular biology, (2011) (PubMed).

    Ito, Batth, Petzold, Redding-Johanson, Mukhopadhyay, Verboom, Meyer, Millar, Heazlewood: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).

  • Target

    VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

    Andere Bezeichnung

    VDAC1

    Hintergrund

    AGI Code: At3g01280
    VDAC1 protein (called also Synonymes: At3g01280, outer mitochondrial membrane protein porin 1, T22N4_9, T22N4.9, VDAC 1, Voltage-dependent anion-selective channel protein 1, voltage-gated ion-selective channel) forms a channel through the cell membrane for diffusion of small hydrophilic molecules. Evolutionary origin of VDAC protein is not clear and their structure and properties are quite different making those proteins only conceptually like porins (Clausen et al. 2004).

    Molekulargewicht

    29 kDa (for Arabidopsis thaliana)

    UniProt

    Q9SRH5
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