VDAC1 Antikörper

Produktnummer ABIN3155731

Zitiert in 5+ Publikationen. Der Kaninchen Polyklonal anti-VDAC1 Antikörper (ABIN3155731) detektiert spezifisch VDAC1 in WB und BN PAGE. Dieser Antikörper reagiert spezifisch mit Proben aus Arabidopsis thaliana, Oryza sativa, Papaver, Physcomytrium Patens, Spinach, Mais (Zea mays), Orange, Fortunella margarita, Cauliflower (Brassica oleracea var. botrytis), Brassica rapa, Beet und Amaranthus palmeri.

Kurzübersicht für VDAC1 Antikörper (ABIN3155731)

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Reaktivität: Arabidopsis thaliana, Oryza sativa, Papaver, Physcomytrium Patens, Spinach, Mais (Zea mays), Orange, Fortunella margarita , Cauliflower (Brassica oleracea var. botrytis), Brassica rapa, Beet, Amaranthus palmeri

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VDAC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Blue-native PAGE (BN PAGE)

Produktdetails anti-VDAC1 Antikörper

Verwendungszweck: Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5

Kreuzreaktivität (Details): Arabidopsis thaliana, Amaranthus palmeri, Beta vulgaris, Brassica oleracea var. botrytis, Brassica rapa subsp. rapa, Citrus sinensis, Fortunella margarita Swingle, Oryza sativa, Papaver sp. pollen tubes (IL), Spinacia oleracea, Physcomitrium patens, Zea mays
Not reactive in: Chlamydomonas reinhardtii, Glycine max, diatoms, Saccharomyces cerevisiae

Homologie: Arabidopsis alpina,Aundo donax,Brachypodium distachyon,Brassica campestris,Brassica napus,Brassica rapa subsp. pekinensis,Capsella rubella,Citrus clementina,Eutrema salsugineum,Glycine max,Glycine soja,Gossypium arboreum,Hoedum vulgare var. distichum,Jatropha curcas,Medicago truncatula,Mesembryanthemum crystallinum,Morus notabilis,Nicotiana tabacum,Phaseolus coccineus,Phaseolus vulgaris,Pisum sativum,Plantago major,Prunus persica,Ricinus communis,Solanum lycopersicum,Solanum tuberosum,Sorghum bicolor,Theobroma cacao,Triticum aestivum,Vitis vinifera

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide conserved in all known higher plant VDAC proteins including Arabidopsis thaliana VDAC1 UniProt: Q9SRH5

Anwendungsinformationen

Applikationshinweise: 1 : 500 (IL), 1 : 5000, 2-30 μg protein/lane (WB)

Kommentare:

Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are available hereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-VDAC1 Antikörper (ABIN3155731)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Barreto, Okura, Neshich, Maia, Arruda: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).

Zhou, Zhao, Yang, Chen, Liu, Jin, Chen, Li, Deng, Schumaker, Guo: "Ubiquitin-specific protease16 modulates salt tolerance in Arabidopsis by regulating Na(+)/H(+) antiport activity and serine hydroxymethyltransferase stability." in: The Plant cell, Vol. 24, Issue 12, pp. 5106-22, (2013) (PubMed).

Takahashi, Teranishi, Ishida, Kawasaki, Takeuchi, Yamaya, Watanabe, Makino, Hidema: "CPD photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA." in: The Plant journal : for cell and molecular biology, (2011) (PubMed).

Ito, Batth, Petzold, Redding-Johanson, Mukhopadhyay, Verboom, Meyer, Millar, Heazlewood: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).

Details zu VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Andere Bezeichnung: VDAC1

Hintergrund:

Background: VDAC proteins are porin-type, beta-barrel diffusion pores. Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.

Additional information: Cellular [compartment marker] of mitochondrial outer membrane for western blot,

Molekulargewicht: 29 kDa (for Arabidopsis thaliana)

Gen-ID: 820914

UniProt: Q9M2W6-1, Q9SMX3-1, F4K3R8-1