PAR1 Antikörper (AA 27-102)

Produktnummer ABIN307496

Der Ziege-Polyklonal Anti-PAR1-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet zum Nachweis von PAR1 in Proben aus Human. }

Kurzübersicht für PAR1 Antikörper (AA 27-102) (ABIN307496)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PAR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-PAR1 Antikörper

Bindungsspezifität: AA 27-102

Spezifität: Selected for its ability to recognize human PAR1 in the applications listed.

Aufreinigung: Affinity purified

Immunogen: E. coli-derived recombinant human PAR1 (aa27-102, 375-428).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human PAR1. The detection limit for rhPAR1 is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody was tested in flow cytometry using whole blood granulocytes. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human PAR1. The detection limit for rhPAR1 is approximately 0.2 ng/well.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Details zu PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Andere Bezeichnung: F2R / Thrombin Receptor / PAR1

Hintergrund: Name/Gene ID: F2R
Subfamily: Proteinase-activated
Family: GPCR

Synonyms: F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1

Gen-ID: 2149

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus