GAL-4, DNA Binding Domain (full length) Antikörper

Produktnummer ABIN307169

Der Maus Monoklonal anti-GAL-4, DNA Binding Domain Antikörper wird verwendet zum Nachweis von GAL-4, DNA Binding Domain in Proben von Saccharomyces cerevisiae. Er wurde validiert für WB und IP.

Kurzübersicht für GAL-4, DNA Binding Domain (full length) Antikörper (ABIN307169)

Target: GAL-4, DNA Binding Domain

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 8C-1

Produktdetails

Bindungsspezifität: full length

Verwendungszweck: Mouse anti Yeast GAL-4 DNA Binding Domain

Kreuzreaktivität: Saccharomyces cerevisiae

Produktmerkmale: Binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

Aufreinigung: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Recognizes a 106 kDa protein by Western blot in yeast cell extracts corresponding to amino acids 1-147 of the GAL4 DNA binding region. Western blot at 1-10 μg/mL. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Antigendetails

Target: GAL-4, DNA Binding Domain

Andere Bezeichnung: GAL-4 DNA Binding Domain

Hintergrund: Mouse anti GAL-4 DNA Binding region, gal4, gal 4 yeast