GAL4 Activation Domain Antikörper

Produktnummer ABIN307168

Dieser Maus Monoklonal Antikörper detektiert spezifisch GAL4 Activation Domain in WB und IP. Es zeigt Reaktivität gegenüber Proben von Saccharomyces cerevisiae.

Kurzübersicht für GAL4 Activation Domain Antikörper (ABIN307168)

Target: GAL4 Activation Domain

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 5C8-5

Produktdetails

Kreuzreaktivität: Saccharomyces cerevisiae

Produktmerkmale: GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

Aufreinigung: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Antibody can be used for Western Blotting (5-10 μg/mL). Recognizes GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Antigendetails

Target: GAL4 Activation Domain

Andere Bezeichnung: GAL-4 Activation Domain