Fibronectin Antikörper

Produktnummer ABIN3071577

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Fibronectin in WB, IHC, ELISA und InhA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Fibronectin Antikörper (ABIN3071577)

Target: Fibronectin

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibronectin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Inhibition Assay (InhA)

Klon: FN12-8

Produktdetails anti-Fibronectin Antikörper

Verwendungszweck: Anti-Human Fibronectin Mouse Monoclonal Antibody

Spezifität: This antibody recognizes an epitope in the cell-binding domain of human and bovine fibronectin and can inhibit cell adhesion.

Kreuzreaktivität: Rind (Kuh), Human

Immunogen: Human plasma fibronectin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 1-10 μg/mL with immobilized antigen.Western Blot: 1-10 μg/mL, reducing or non-reducing conditions.Cell Adhesion Inhibition Assay: 0.1-1.0 μg/mL on 10 μg/mL fibronectin-coated microwells inhibits adhesion of trypsinized NRK cells.
Immunohistochemistry: 1-10 μg/mL, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval.Staining of fibronectin (FN) in human well-differentiated colon cancer tissue (bottom) and moderately- differentiated colon cancer (top) with #42040.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute lyophilized antibody in 200 µL of distilled water (final concentration 2.0 mg/mL).

Buffer: PBS, pH 7.4, 1 % BSA, lyophilized.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The stock solution (2. 0mg/ml) can be stored in aliquots at -20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Prepare dilutions of stock solution immediately before use. Do not store diluted stock solution. Avoid repeated freeze-thaw cycles

Details zu Fibronectin

Target: Fibronectin

Hintergrund: Fibronectin,Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). {UniProtKB:P11276, PubMed:3024962, PubMed:34089617, PubMed:3593230, PubMed:3900070, PubMed:7989369}., [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. {PubMed:11209058, PubMed:15665290, PubMed:19379667, PubMed:8114919}.,Secreted, extracellular space, extracellular matrix,FN, Cold-insoluble globulin, CIG [Cleaved into: Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3]

UniProt: P02751