HNRNPK Antikörper (C-Term)

Produktnummer ABIN307124

Der Maus Monoklonal Anti-HNRNPK-Antikörper wurde für WB und IHC validiert. Er ist geeignet, HNRNPK in Proben von Human zu detektieren.

Kurzübersicht für HNRNPK Antikörper (C-Term) (ABIN307124)

Target: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HNRNPK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: F45 P9 C7

Produktdetails anti-HNRNPK Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität: Huhn, Human, Maus, Kaninchen, Ratte

Produktmerkmale: hnRNP-K, CSBP, TUNP, dC-Stretch Binding Protein,Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.

Aufreinigung: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the hnRNP-K protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse, rat, rabbit and chicken

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu HNRNPK

Target: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Andere Bezeichnung: Heterogeneous Nuclear Ribonucleoprotein K

UniProt: P61978