Estrogen Receptor alpha Antikörper

Produktnummer ABIN306907

Dieser Maus Monoklonal Antikörper detektiert spezifisch Estrogen Receptor alpha in WB, IP, ChIP, GS und InhA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Kaninchen, Schwein, Hund, Huhn, Hamster und Schaf.

Kurzübersicht für Estrogen Receptor alpha Antikörper (ABIN306907)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Schwein, Hund, Huhn, Hamster, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Estrogen Receptor alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Inhibition Assay (InhA)

Klon: TE111-5D11

Produktdetails anti-Estrogen Receptor alpha Antikörper

Verwendungszweck: Mouse anti Human Estrogen Receptor

Kreuzreaktivität: Huhn, Rind (Kuh), Hund, Hamster, Human, Maus, Schwein, Kaninchen, Ratte, Schaf

Produktmerkmale: The estrogen receptor (ER) gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Aufreinigung: Protein A/G Chromatography

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: This antibody can be used gel supershift assays (neat concentration), immunoprecipitation on native and denatured lysate (2 μg/mg of protein lysate), Western blotting (1-2 μg/mL) and chromatin precipitation. Can also be used to block binding of estrogen to the estrogen receptor. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Andere Bezeichnung: Estrogen Receptor

UniProt: P11474

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly