ELISA, Western Blotting (WB), Flow Cytometry (FACS)
Klon
F4-1
Verwendungszweck
Mouse anti FITC (Fluorescein isothiocyanate)
Produktmerkmale
The F4/1 antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxyfluorescein. Sensitivity of the FITC-anti- FITC system is reportedly similar to that of the biotin-avidin system. When conjugated to biotin or enzyme-labeled, this antibody is also suitable for the conversion of fluorescein-labeled detection into immunoenzymatic assays.
This antibody can be used for Western blotting (1-2 μg/mL), immunohistochemistry (frozen and formalin/paraffin tissues, 2-4 μg/mL) and flow cytometry. Optimal concentration should be evaluated by serial dilutions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.95 mg/ml
Buffer
phosphate buffered saline with 0.08 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles