Lysophospholipid Phosphatase (LPP) 2 Antikörper

Produktnummer ABIN306893

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für Lysophospholipid Phosphatase (LPP) 2 Antikörper (ABIN306893)

Target: Lysophospholipid Phosphatase (LPP) 2

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB)

Produktdetails

Kreuzreaktivität: Human

Produktmerkmale: Lipid phosphate phosphohydrolase 2, EC 3.1.3.4, Phosphatidic acid, phosphatase 2c, PAP-2c, PAP2c, Phosphatidate phosphohydrolase type 2c, PAP2-gamma, PAP2-G,Phosphatidic acid phosphatase type 2 (PAP2) was originally identified as a plasma membrane enzyme that catalyses the dephosphorylation of the putative second messenger, phosphatidic acid (PA) to diacylglycerol (DG) [1]. Subsequently, multiple isoforms of PAP2 were cloned [2-5]. It was found that these enzymes dephosphorylate a number of lipid phosphates in vitro other than PA, including the potent bioactive lipids, lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P). Therefore, they have been renamed lipid phosphate phosphatases (LPPs). Currently, there are four members of this family called LPP1, LPP1a, LPP2 and LPP3 [6]. S1P [7] and LPA [8] regulate the proliferation, differentiation, apoptosis and migration of cells by binding to a family of G protein-coupled receptors. Thus, EDG1/S1P1, EDG3/S1P3, EDG5/S1P2/AGR16/H218, EDG6/S1P4 and EDG8/S1P5/nrg-1 are high affinity S1P receptors [7] whereas EDG2/LPA1, EDG4/LPA2 and EDG7/LPA3 have high affinity for LPA [8]. Recently, the over-expression of LPP1 was shown to limit LPA-stimulated signalling in Rat2 fibroblasts [9] and LPA-stimulated DNA synthesis in HEK 293 cells [10]. Similarly, over-expression of LPP1, LPP1a and LPP2 attenuate S1P-signalling to the p42/p44 mitogen activated protein kinase cascade [11].

Aufreinigung: Ammonium Sulfate Precipitation

Immunogen: Rabbits were immunized with a unique peptide derived from the human lysophospholipid phosphatase 2 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This antibody can be used for Western blotting (10-15 μg/mL). Optimal concentration should be evaluated by serial dilutions. NOTE: Boiling LPP2 with sample buffer will aggregate the protein. Lysates should be prepared by mixing cells with lysis buffer (possibly with extra detergent) to solubilize the protein before adding sample buffer and lysate SHOULD NOT be boiled/heated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Antigendetails

Target: Lysophospholipid Phosphatase (LPP) 2