P34 Cdc-2 Nuclear Protein Antikörper

Produktnummer ABIN306818

Der Maus Monoklonal anti-P34 Cdc-2 Nuclear Protein Antikörper wird verwendet zum Nachweis von P34 Cdc-2 Nuclear Protein in Proben von Rind (Kuh), Human, Maus, Ratte und Xenopus laevis. Er wurde validiert für ICC, IHC (p), IP und WB.

Kurzübersicht für P34 Cdc-2 Nuclear Protein Antikörper (ABIN306818)

Target: P34 Cdc-2 Nuclear Protein

Reaktivität: Rind (Kuh), Human, Maus, Ratte, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Klon: POH-1

Produktdetails

Verwendungszweck: Mouse anti Human p34 cdc-2 nuclear protein

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte, Xenopus laevis

Produktmerkmale: p34(Cdc2) kinase is a an essential nuclear protein for mitosis in human cells. During the prophase/metaphase transition the inactive phosphorylated form of p34(cdc2) is dephosphorylated to make the active form of the protein. This dephosphorylation is performed by the p80(cdc25) dual-specificity phosphatase. The regulatory subunit of p34(cdc2) is cyclin B, a 47-52 kDa protein. The p34(cdc2) protein is the catalytic subunit of the M phase-promoting factor (MPF) which is intracellular factor responsible for entry into M phase. Three mechanisms are responsible for the activitation of MPF at the onset of mitosis: binding of p34(cdc2) to cyclin B, a series of phosphorylation/dephosphorylation events on the two subunits and translocation of the complex to the nucleus or microtubules.

Aufreinigung: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Detects three bands within the 34 kDa region corresponding to p34 and its cleavage products. Can also be used for IHC on paraffin-embedded tissue (10 μg/mL) as well as immunocytochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Antigendetails

Target: P34 Cdc-2 Nuclear Protein

UniProt: P06493