RIT1 Antikörper

Produktnummer ABIN306804

Dieses Anti-RIT1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von RIT1 in WB. Geeignet für Human.

Kurzübersicht für RIT1 Antikörper (ABIN306804)

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RIT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 14G7

Produktdetails anti-RIT1 Antikörper

Kreuzreaktivität: Human, Maus

Produktmerkmale: Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.

Aufreinigung: Protein A or G purified

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant full-length human Rit protein and mouse myeloma cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Antibody detects recombinant Rit protein expressed in 293 cells by Western blot analysis at a concentration of 0.5 to 1 μg/mL. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa). Does not cross-react with Rin protein. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50 % glycerol at pH 7.5 and 3 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu RIT1

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Andere Bezeichnung: Rit

UniProt: Q92963

Pathways: Neurotrophin Signalübertragung